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Site developer and 1st Year PhD Student (Biomedical Research) at the University of Birmingham
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A Salting Out Procedure for DNA extraction from cheek cells obtained by rinsing the mouth with 25mls of any commercial mouth wash solution available for about 30sec the first thing in the morning. It is important not to brush your teeth before collecting the specimen.
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Media for freezing of cells for culture.
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Analyzing a dot blot in ImageJ by background subtraction and measuring the integrated density of each dot.
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Analyzing a dot blot in ImageJ treating each horizontal "lane" and use ImageJ's gel analysis function.
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This is an example of how to measure the lengths of DNA contours on images acquired using an atomic force microscope (AFM).
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31
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100 mM Sodium orthovanadate
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2
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69
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Basic outline protocol for immunohistochemistry on unfixed cytostat-thin tissue sections
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Purification of EBV viral particles via consecutive ultracentrifugation in horizontal and vertical centrifuges.
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B cell enrichment using Invitrogen Dynabeads magnetic separation
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Restoration of culture cells from storage at -80°C with 10% DMSO cryopreservation.
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Differentiation of HL-60 to neutrophil or monocyte-like cells by Vitamin A (ATRA) and Vitamin D (1a,25(OH)2D3)
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536
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GM-CSF/M-CSF differentiation of CD14+ PBMCs to M1/M2 macrophage subsets.
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Primary monocyte differentiation to DCs with GM-CSF and IL-4
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Isolation of CD14+ subset of monocytes by MACS magnetic bead isolation.
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Silanized (3-aminopropyltriethoxysilane) slides to prevent sections from detaching.