How do you isolate lipoprotein

I would like to isolate LDL, and HDL and subject them to gradient electrophresis for particle size determination.I am just wonder if there is any one who has done this before. I have human plasma and serum samples. According to some paper that i read, the protocol involves adjustment of the density before ultracentrfugation with Kbr.However,, i do not know how to adjust density of plasma before fractionation. I really appreciate your help. If you could please be detailed as much as you can.

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  +1  

According to the attached reference, you can adjust the density of the plasma for centrifugation by the addition of 1.33g/ml of KBr.

In the paper which I think you're referring to here it gives the target density as 1.30g/ml, suggesting that the plasma itself must have a low to zero density. Although the attached paper references this group for the method, it provides a bit more practical detail.

Bowry VW, Stanley KK, Stocker R High density lipoprotein is the major carrier of lipid hydroperoxides in human blood plasma from fasting donors. Proc Natl Acad Sci U S A Jan. 1, 1992

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Martin Fitzpatrick

3 months ago

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