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Basic protocol for Eosin & Haematoxylin histological preparation of microdissection slide.
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Immunoprecipitation protocol for use with C. elegans worm lysate
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Purification of 6XHIS proteins with cell extraction buffer
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In-gel Tryptic Digest for Protein ID by Mass Spectrometry
(David Miyamoto, 2/12/2002). This protocol is based on Shevchenko A, Wilm M, Vorm O, & Mann M. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal Chem 1996, 68:850-8. I have used it with success on both Coomassie and silver-stained gel bands.
