- Immunohistochemistry (3)
- Fixation (11)
- Staining (6)
2
votes
69
views
2
users
Basic outline protocol for immunohistochemistry on unfixed cytostat-thin tissue sections
2
votes
43
views
1
users
Staining protocol for C. Elegans embryos.
2
votes
9
views
1
users
Antibody diluent for IHC
1
votes
27
views
1
users
Basic protocol for Eosin & Haematoxylin histological preparation of microdissection slide.
1
votes
172
views
1
users
Fixation for flow cytometry with ethanol. This method is very simple, quick and
allows samples to be stored for weeks at –20°C.
1
votes
4
views
1
users
Immunohistochemistry (cultured cells and monolayers)
1
votes
10
views
1
users
Luxol Fast Blue histological stain. This technique is a method requiring over-staining of tissues and differentiation to reveal the myelin. It is advisable to include a control showing de-myelination pathology to ensure the end point of staining is reached. Cresyl Fast Violet counter-stain is optional and results in neuronal piliform staining and a deepening of the myelin blue staining.
0
votes
40
views
1
users
Silanized (3-aminopropyltriethoxysilane) slides to prevent sections from detaching.
0
votes
24
views
1
users
Fixative for smears, cytospin preparations and frozen sections
0
votes
20
views
1
users
Fixation of histological samples with Bouin's fluid
0
votes
35
views
1
users
Fixation of samples in Carnoy's fluid
0
votes
19
views
1
users
Fixation in formol sublimate
0
votes
29
views
1
users
Fixation using Helly's fluid
0
votes
28
views
1
users
Fixation with neutral buffered formalin
0
votes
54
views
1
users
Michel's fixative for immunofluorescence
0
votes
15
views
1
users
Fixation with paraformaldehyde
0
votes
22
views
1
users
Fixation with Zenker's fluid
0
votes
37
views
1
users
Alternative fixation procedure for FACS fixation, using formaldehyde fixatice.
0
votes
7
views
1
users
0
votes
35
views
1
users
Immunostaining of Cells Adherent to Coverslips
