Cresyl

Luxol Fast Blue histological stain. This technique is a method requiring over-staining of tissues and differentiation to reveal the myelin. It is advisable to include a control showing de-myelination pathology to ensure the end point of staining is reached. Cresyl Fast Violet counter-stain is optional and results in neuronal piliform staining and a deepening of the myelin blue staining.

Cresyl Violet Staining for paraffin embedded sections. Cresyl Violet Acetate solution is used to stain Nissl substance in the cytoplasm of neurons in paraformaldehyde or formalin-fixed tissue. The neuropil will be stained a granular purple-blue. This stain is commonly used to identify the neuronal structure in brain and spinal cord tissue.

Cresyl Violet Staining for free floating sections, mounted and air-dried. Cresyl Violet Acetate solution is used to stain Nissl substance in the cytoplasm of neurons in paraformaldehyde or formalin-fixed tissue. The neuropil will be stained a granular purple-blue. This stain is commonly used to identify the neuronal structure in brain and spinal cord tissue.