About
DAPI counterstain for chromosome FISH analysis
Method
30 minutes
None
30 minutes
1
After FISH sample preparation briefly rinse sample in distilled water to remove residual salts
2
Dilute stock DAPI to a concentration of 30nM in PBS
3
Add 300ul of diluted DAPI solution directly onto the sample, ensuring sample is covered equally
4
Incubate at room temperature for 30 minutes in the dark
30 minutes
Wait for
•
29 minutes, 59 seconds
5
Rinse specimen with distilled water or PBS to remove DAPI solution
6
Blot edge of slide with tissue to remove excess water/solution from the side
7
Mount slide with appropriate slide mountant, nail polish or wax.