About
8% Paraformaldehyde for Immunofluorescence
Method
30 minutes
30 minutes
None
1
Add 16g paraformaldehyde to approximately 160ml water in fume hood.
2
Heating on setting 4.5 whilst stirring to 50ºC.
3
Once temperature reaches 50ºC, 1M NaOH until the paraformaldehyde is just dissolved.
4
Make up to final volume of 200ml with water, pH to 7.2-7.4.
5
Filter with 0.22µm filter.
6
Aliquot in 5ml tubes, label and freeze.