- Histology (29)
-
Molecular biology
(38)
- Rna,
- Dna,
- Pcr,
- Xli-blue,
- Pbluescript
- Model organisms (7)
- Microbiology (6)
- Immunology (9)
- Imaging (9)
- Cell biology (10)
- Media & solutions (39)
- Biochemistry (5)
- Neuroscience (1)
2
votes
69
views
2
users
Basic outline protocol for immunohistochemistry on unfixed cytostat-thin tissue sections
2
votes
45
views
1
users
A generic ELISA protocol with optional quantitative step.
2
votes
28
views
2
users
Recipe for 10x Tris Buffer, dilute for use
2
votes
43
views
1
users
Staining protocol for C. Elegans embryos.
2
votes
9
views
1
users
Antibody diluent for IHC
2
votes
9
views
1
users
Easy to use, add 2ul per 10ul of DNA solution.
1
votes
42
views
1
users
B cell enrichment using Invitrogen Dynabeads magnetic separation
1
votes
58
views
1
users
Restoration of culture cells from storage at -80°C with 10% DMSO cryopreservation.
1
votes
112
views
1
users
Differentiation of HL-60 to neutrophil or monocyte-like cells by Vitamin A (ATRA) and Vitamin D (1a,25(OH)2D3)
1
votes
536
views
1
users
GM-CSF/M-CSF differentiation of CD14+ PBMCs to M1/M2 macrophage subsets.
1
votes
30
views
1
users
Isolation of CD14+ subset of monocytes by MACS magnetic bead isolation.
1
votes
52
views
1
users
1
votes
27
views
1
users
Basic protocol for Eosin & Haematoxylin histological preparation of microdissection slide.
1
votes
172
views
1
users
Fixation for flow cytometry with ethanol. This method is very simple, quick and
allows samples to be stored for weeks at –20°C.
1
votes
362
views
1
users
Determining the level of cellular fluorescence from fluorescence microscopy images in ImageJ
1
votes
46
views
2
users
Paper strip method for DNA extraction from agarose gels
1
votes
41
views
2
users
1
votes
31
views
1
users
The preparation, and running, of an agarose gel for nucleic acid separation.
1
votes
45
views
1
users
Ligation of a DNA fragment to a vector
1
votes
16
views
1
users
Isolate a DNA fragment from Low Melting agarose